How to add genetic parts to GenoCAD?

add genetic parts

In the previous video, we learned how to create a genetic grammar in GenoCAD. In this video, we will add genetic parts to this grammar.

Adding genetic parts to GenoCAD is easy. There are three main ways. The first is to manually add a single part to GenoCAD. Let’s click on “Parts and Grammars” and then the “My Parts” tab. Here we can add a single part using the “New Part” button. You see that no library exists for the Test Grammar we developed in a previous video, so we’ll have to add one before we’re able to add any parts.

Now we can add a part manually. Let’s add an RBS.

The second way to add parts is to import a tab-delimited text file or FASTA file containing many parts, which is much faster than adding parts to GenoCAD one at a time. Let’s import a tab-delimited text file. We have four columns. Left to right, they are:

  1. The part name
  2. The category to which it belongs
  3. A short description of the part and
  4. The DNA sequence of the part.

Notice that headers are not permitted.

After we import the file, the parts have been automatically added to the correct category.

In Excel, it’s important to save this file as a Windows Formatted Text file. In a standard text editor, save it as a UTF-8 encoded text file. GenoCAD plays nicely with these file formats.

The third way to add parts is to simply import an existing GenoCAD grammar. The grammar files contain the associated parts libraries and parts sequences.

And that’s how easy it is to add parts to GenoCAD. In the next video, we will learn how to use these parts in combinatorial experimental designs.