CMV-T7 RNAP
CMV-T7 RNAP
SKU:TV-MCEv1-T7RN-BKGQH-0ZM7H-1
TrueVector-CMV-T7RNAP (AmpR, High Copy) is a high-yield expression plasmid designed for strong, transient expression of T7 RNA polymerase in mammalian cells. It uses a CMV-derived promoter for robust transcription and is codon-optimized for H. sapiens.
This plasmid is ideal as a helper vector in systems that require T7-driven transcription, such as the recovery of RNA viruses or the expression of T7-controlled transgenes.
Applications: Transient expression and Control
Expression: Consititutive
Gene: T7 RNA Polymerase
Codon Optimization: H. sapiens
Host: Mammalian cell lines
Selection markers: Ampicilin / Carbenicillin (E. coli)
Copy number: High-copy number
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This TrueVector™ Plasmid is part of the GenoFAB® TrueVector™ collection, designed for data-driven synthetic biology workflows.
Key Features
- Flexible Formats: Available as transfection-ready plasmid DNA or CorePrep™, a streamlined format free of bacterial sequences.
- Customizable Expression: Compatible with plasmids like TV-MCEv1-CUST-VHTQJ-07U93, tailored to express your gene of interest.
- Self-Documenting Design: Integrates patented GSIN™ technology, embedding a digital certificate with:
- Unique serial number for traceability
- Digital signature and error-correcting codes for secure plasmid exchange
What’s Included
- DNA Format: Lyophilized, transfection-grade DNA for enhanced stability during shipping
- Resuspension Options: Reconstitute in nuclease-free water or TE buffer
- Minimum Yield: ≥100 µg
- Endotoxin Level: <0.1 EU/µg (ultra-low endotoxin)
- Documentation: Access a unique URL with:
- GenBank, SnapGene, and Geneious files
- Licensing agreement
- User manual
- Quality control report
Quality Control Every TrueVector plasmid undergoes rigorous QC, including:
- DNA Sequencing: Verifies sequence integrity post-amplification
- Spectrophotometry: Measures purity (A260/280/230)
- Endotoxin Testing: Ensures compatibility with sensitive preclinical workflows